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4fb9d08492 doi: 10.1371/journal.pntd.0000577 pmid:20052267; PubMed Central PMCID: PMC2791843. doi: 10.1186/1471-2334-12-8 pmid:22257432; PubMed Central PMCID: PMC3398261. The plate was developed in chloroform: hexane: methanol at a ratio of 5:4:1 until the leading edge reached the top of the plate, air-dried and dipped in 0.1 M 2-naphthylboronic acid solution in acetone, then heated for 60 seconds at 100C on a hot plate. The specificity was 87% but the implication of four false positives is that 4 patients would have received treatment for 8 weeks with daily injections of streptomycin and oral rifampicin with the known potential for side effects associated with those drugs. Epub 2007/06/08. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Competing interests: The authors have declared that no competing interests exist. WHO. 2009;47(4):9246.
As PCR is sophisticated and expensive its use has been restricted to a small number of reference laboratories  and numerous studies have been carried out to develop a simple diagnostic test that can be used at point of care facilities  . Epub 2010/02/18. Accelerated detection of mycolactone production and response to antibiotic treatment in a mouse model of Mycobacterium ulcerans disease. Advertisement Archived Tweets Load more View all tweets . We are sorry, but an unhandled error occurred. doi: 10.1186/1471-2334-14-202 pmid:24731247; PubMed Central PMCID: PMC4021496. Standard routine laboratory techniques for the confirmation of Buruli ulcer disease are M. Converse PJ, Xing Y, Kim KH, Tyagi S, Li SY, Almeida DV, et al.